1. 负责人简介

    朱鹏，国科宁波生命与健康产业研究院研究员，宁波市领军和拔尖人才“第三层次”。 主持国家级项目1项，省级项目2项，宁波市科技局重点项目1项，以第二完成人参与国家级项目2项，省部级项目3项，市科技局项目3项。同时，作为核心成员参与省创新团队和市科技创新团队各1个。近5年来先后发表论文30篇，SCI收录16篇；申请发明专利13项，授权8项；参与制定军用标准1项；获宁波市科技进步奖三等奖3项，宁波市自然科学优秀论文三等奖1项。

2. 研究方向

1）分子诊断与检测技术开发。现专注基于组学技术的消化系统肿瘤新型诊断标志物的挖掘及临床诊断价值的研究。

2）中药有效成分成药性研究。现专注甾体皂苷类化合物在消化道肿瘤预防/治疗中的研究

3. 发表论著

  [1]. Peng Zhu, Zouan Huang, Zi Xiong, Shiyu Guo, Shun Zhang, Ting Cai. Development and evaluation of real-time recombinase polymerase amplification assay for rapid and sensitive detection of Vibro mimicus in human plasma samples. Journal of Applied Micobiology. 2022. 133(3): 1650-1659.

  [2]. Ling Hu, Yidong Fu, Shun Zhang, Zhilei Pan, Jiang Xia, Peng Zhu*, Jing Guo. An assay combining droplet digital PCR with propidium monoazide treatment for the accurate detection of live cells of Vibrio vulnificus in plasma samples. Frontiers in Microbiolog. 2022. 13: 927285.

[3]. Peng Zhu, Yan Cui, Jianhu Pang, Zi Xiong, Zuoan Huang, Shiyu Guo, Shun Zhang, Ting Cai. Sensitively and quickly detecting Vibrio vulnificus by real time recombinase polymerase amplification targeted to vvhA gene. Molecular and Cellular Porbes. 2021, 57: 101726.

[4]. Jianhu Pang, Qiong Wang, Yuejun Fei, Peng Zhu*, Longliang Qiao, Hailong Huang, Chenyang Dang, Weifang Gao. A real-time recombinase polymerase amplification assay for the rapid detection of Vibrio harveyi. Molecular and Cellular Porbes. 2019, 44: 8-13.

[5]. Peng Zhu, Weifang Gao, Hailong Huang, Jinpo Jiang, Xianfeng Chen, Jianzhong Fan, Xiaojun Yan. Rapid Detection of Vibrio parahaemolyticus in Shellfish by Real-Time Recombinase Polymerase Amplification. Food Analytical Methods. 2018, 11(8) : 2076–2084.

[6]. Weifang Gao, Hailong Huang, Peng Zhu*, Xiaojun Yan, Jianzhong Fan, Jinpo Jiang, Jilin Xu. Recombinase polymerase amplification combined with lateral flow dipstick for equipment-free detection of Salmonella in shellfish. 2018, 41:603–611.

[7]. Weifang Gao, Hailong Huang, Yan Zhang, Peng Zhu*, Xiaojun Yan. Recombinase polymerase amplification – based assay for rapid detection of Listeria monocytogenes in food samples. Food Analytical Methods, 2017, 10: 1972-1981.

[8] Huang HL, Peng Zhu*, Zhou CX, He S, Yan XJ. The development of loop-mediated isothermal amplification combined with lateral flow dipstick for detection of Karlodinium veneficum. 2017, 62: 20-29.

[9]. Huang HL, Peng Zhu*, Zhou CX, Yan XJ, Zou YX, Lv PW. Detection of Skeletonema costatum based on loop-mediated isothermal amplification combined with lateral flow dipstick. Molecular & Cellular Probes. 2017,36:36-42.

[10] Qiao LL, Peng Zhu*, Pang JH. Type II Thioesterases And Its Role In The Synthesis Of Secondary Metabolite. Chinese Journal of Biochemistry and Molecular Biology. 2017, 33(11):1115-1124.

[11]. Fang J, Jian HX, Peng Zhu*. Integrin and Its Inhibitor Design. Chinese Journal of Biochemistry and Molecular Biology. 2017, 33(6):553-540.

[12]. Qiao LL, Pang JH, Peng Zhu*. CRISPR/Cas9 Genome Editing Technology and Its Application in Streptomyces. Biotechnology Bulletin.2017 (Accepted).

[13]. Gao WF, Huang HL, Zhang Y, Peng Zhu*, Yan XJ, Fan JZ, Chen XF. Recombinase Polymerase Amplification-Based Assay for Rapid Detection of Listeria monocytogenes in Food Samples. Food Anal. Methods. 2017, 10: 1972-1981.

[14]. He KF, Gao WF, Fang J, Wang JQ, Li LY, Peng Zhu*. Analysis of the Applicability of Peptides Cyclization Catalyzed by Cyanophyta Thioesterase. Chinese Journal of Biochemistry and Molecular Biology. 2017, 33(1): 88-97.

[15]. Fang F, Zhao J, Ding LJ, Huang C, Naman CB, Wu B, Peng Zhu, He S. 5-Hydroxycyclopenicillone, a New β-Amyloid Fibrillization Inhibitor from a Sponge-Derived Fungus Trichoderma sp. HPQJ-34. Marine Drugs, 2017, 15(8):260.

[16]. Xiong Y, Yang H, Peng Zhu*, Huang HL. Rapid and Sensitive Detection of the Main Contaminating Fungus Penicillium restrictum in Jet Fuel Using Loop-mediated Isothermal Amplification Combined with a Lateral Flow Dipstick. 2016, 20(2): 301-312.

[17]. Hao Y, Yun X, Peng Zhu*, Chen R, Huang HL and He QQ. Studies of influence on the physicochemical properties of jet fuel by Aspergillus penicillioides. Journal of Chemical and Pharmaceutical Research, 2016, 8(4):468-471.

[18]. Li L, He S, Ding L, Yuan Y, Peng Zhu, Epstein S, et al. Efficient preparation of streptochlorin from marine streptomyces sp. syylwhs-1-4 by combination of response surface methodology and high-speed counter-current chromatography. Molecules, 2016, 21(6), 693.

[19]. Zhou Y, Huang H, Li XJ, Shan CL, Li XS, Chen Y, Shao W, Peng Zhu. Establishment of Leptosphaeria maculans detection by loop-mediated isothermal amplification combined with a lateral -flow dipstick. PLANT QUARANTINE, 2016. 30(4): 32-37.

[20]. Xiong Y, Yang H, Peng Zhu, Huang HL, He QQ, Zhou J. Repaid and Accurate Detection of Khuskia oryzae by Loop- mediated isothermal Amplification Combined with a Lateral Flow Dipstick. Chemical Engineering Transactions. 2015, 46 877-882.

[21]. Jin H X, Go M L, Yin P, Qiu XT, Peng Zhu, Yan X J. Determining the functions of hiv-1 tat and a second magnesium ion in the cdk9/cyclin t1 complex: a molecular dynamics simulation study. Plos One, 2015,10(4), e0124673.

[22]. Gu B B, Zhang Y Y, Ding L J, He S, Wu B, Dong J D, Peng Zhu, Chen JJ, Zhang J R, Yan XJ. Preparative separation of sulfur-containing diketopiperazines from marine fungus cladosporium sp. using high-speed counter-current chromatography in stepwise elution mode. Marine Drugs, 2015,13(1), 354-365.

[23]. Peng Zhu, Zhang BF, Wu JH, Dang CY, LV YT, Fan JZ, Yan XJ. Sensitive and Rapid Detection of Microcystin Synthetase E Gene (mcyE) by Loop-Mediated Isothermal Amplification: A New Assay for Detecting the Potential Microcystin-producing Microcystis in the Aquatic Ecosystem. Harmful Algae. 2014, 37: 8-16.

[24]. Cai BQ, Jin HX, Yan XJ, Peng Zhu, Hu GX. 3d-qsar and 3d-qssr studies of thieno[2,3-d]pyrimidin-4-yl hydrazone analogues as cdk4 inhibitors by comfa analysis. Acta Pharmacologica Sinica, 2014, 35(1): 151.

[25]. Wang TT, He S, Yan XJ, Peng Zhu. Macrolactins: antitumor antibiotics as marine drug lead. Current Organic Chemistry, 2014,18(7), 804-811

[26]. He S, Wang HQ, Yan XJ, Yan XJ, Peng Zhu, Chen JJ, Yang R. Preparative isolation and purification of macrolactin antibiotics from marine bacterium Bacillus amyloliquefaciens using high-speed counter-current chromatography in stepwise elution mode. Journal of Chromatography A, 2013, 1272:15-19.

[27]. He S, Wang HQ, Wu B, Zhou H, Peng Zhu, Yang R, Yan XJ. Response surface methodology optimization of fermentation conditions for rapid and efficient accumulation of macrolactin A by marine Bacillus amyloliquefaciens ESB-2. Molecules, 2013,18: 408-417.

[28]. He S, Wang TT, Yang R, Gu BB, Peng Zhu, Xu YR, Jin HX, Yan XJ. Advances in marine polyphenol analysis. Current Analytical Chemistry,2013, 9(3). 368-373.

[29]. Peng Zhu, Ye YF, Pei FF, Lu KH. Characterizing the structural diversity of a bacterial community associated with filter materials in recirculating aquaculture systems of Scortum barcoo. Canadian Journal of Microbiology, 2012, 58: 303-310.

[30]. Chen W, Peng Zhu*, He S, Jin HX, Yan XJ. Nonribosomal peptides synthetases gene clusters and core domain in Pseudoalteromonas sp. NJ631. Acta Microbiologica Sinica, 2012, 52:1531-1539.

4. 承担科研项目

4.1 国家：

[1]. 共附生交替假单胞菌中次生代谢基因模块结构研究（40906080），国家青年科学基金项目

[2]. 海洋Uncultured放线菌资源挖掘及新天然产物发现（41306134），国家青年科学基金项目

4.1 省部级

[1]. 基于重组酶聚合酶介导的实时等温扩增技术快速检测临床样本中拟态弧菌的方法开发研究（LGF22H190003），浙江省基础公益研究计划

[2]. 利用重组酶介导的等温扩增技术快速检测临床样本中的创伤弧菌（LGC19H190001）, 浙江省基础公益研究计划

[3]. 环介导等温扩增结合横向流动试纸快速检测东海海域中剧毒卡罗藻的试剂盒开发及应用示范（2017C33133）, 浙江省基础公益研究计划

[4]. 主要水产病害检测与免疫预防技术研究与示范（2012C12009-4）,浙江省重大专项

[5]. 鱼糜、鱼粉清洁高值化生产关键技术研发与产业化示范（201305013），国家海洋局公益性行业科研专项

[6]. 宁波海洋生物高值利用公共服务平台（NBHY-2017-P2）国家海洋局“十三五”海洋经济示范城市专项。

[7]. 国家贝类产业技术体系双壳类营养与饲料（CARS-47-3），国家农业部/现代农业产业技术体系项目

4.2 市级

    [1]. 富含脑黄金海洋微生物的筛选及开发应用研究（2015C10026 ），市农业与社会发展攻关项目

[2]. 海洋抗菌微生物产业化研究及其在大黄鱼养殖中的应用示范（2017C10016），市科技富民项目

5. 项目组简介

   项目组成员：

     嵇元烨 硕士 数字液滴PCR扩增技术应用开发

     杨杰民 硕士 等温扩增技术应用开发

     脱萍   硕士 核酸质谱技术应用开发

     韩辉   硕士 甾体皂苷类在消化道肿瘤预防/治疗中的应用研究

     汪萌   硕士 生物信息数据挖掘。